NANOSPACER

TECHNOLOGY

OPEN-SOURCE Tracking software (FIJI)

NTA data handling has never been more transparent. Use freely available tracking software to get most out of your data. Trackmate (FIJI) and Trajectory classifier TraJ (FIJI) plugins allow to analyze your NTA sizing videos free of charge. Analyze EVs, NPs on your own laptop whenever, wherever you like.

NANOSPACER confinement NTA; Smaller fast moving particles are colored in blue, while larger slower particles are marked in red. (Dark-field 50x, Trackmate)

STEP 1

Nanospacer

Obtain microscopy video

STEP 2

STEP 3

Trackmate/ NanoTrackJ

Obtain tracking data (TIFF)

Obtain diffusion coefficient D

Einstein-Stokes

Calculate size... Done!

Unsure about physics? No problem - NANOSPACER AS also offers NTA services and training specific to your application.
Just drop us a message in the contact form and we'll be happy to upgrade your project.

SOFTWARE SOURCES AND REFERENCES

NANOSPACER publications:

Nano-dynamic imaging: NanoSpacer as a low-cost optical method for real-time visualisation of nanoparticle disassembly and functionalisation | Research Square

Andrew Baker, Anna Erdinger et al. , 22 January 2026, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-8389168/v1]

Multimodal imaging of brain metastasis-derived extracellular vesicles | IJN

Feginn Berle B, Juliussen S, Castilho Á, Solel E, Sdik Saed H, Vanderpoorten O, Lunavat TR, Thorsen F, Rigg E. Int J Nanomedicine. 2025 Jun 13;20:7501-7514. doi: 10.2147/IJN.S520791. PMID: 40535836; PMCID: PMC12174924.

TrackMate reference and publication:

Link to Fiji plugin: TrackMate

Ershov, D., Phan, MS., Pylvänäinen, J.W. et al. TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines. Nat Methods 19, 829–832 (2022). https://doi.org/10.1038/s41592-022-01507-1

TraJ reference and publication:

Link to Fiji plugin: TraJClassifier

Wagner T, Kroll A, Haramagatti CR, Lipinski HG, Wiemann M (2017) Classification and Segmentation of Nanoparticle Diffusion Trajectories in Cellular Micro Environments. PLOS ONE 12(1): e0170165

NanoTrackJ reference and publication:

Link to Fiji plugin: NanoTrackJ

Wagner T, Lipinski HG, Wiemann M. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. J Nanopart Res. 2014;16(5):2419. doi: 10.1007/s11051-014-2419-x. Epub 2014 May 1. PMID: 24839395; PMCID: PMC4021069.